Tl1a reporter cell line. Blocking the TL1A-DR3 .
Tl1a reporter cell line. Feb 18, 2025 · A TL1A reporter cell line was employed and treated with escalating doses of anti-TL1A antibody. TL1A stimulation was blocked by the neutralizing anti-TL1A antibody. Blocking the TL1A-DR3 Description TNF-like ligand 1A (TL1A)-Responsive Luciferase Reporter Jurkat Cell Line is a TL1A-responsive DR3/NF-kB luciferase reporter Jurkat cell line expressing firefly luciferase under the control of an NF-kB response element, and with stable expression of human DR3 (death receptor 3; TNFRSF25; NM_003790. Cytokine reporter cells enable detection of these biologically active cytokines Tumor necrosis factor-like ligand 1A (TL1A), or TNFSF15, is a cytokine primarily expressed by endothelial cells. TL1A stimulation was blocked by the neutralizing anti-TL1A antibody (#101729) and DcR3 (decoy receptor 3). Cells were treated with increasing concentrations of TL1A or TNFα for 5 hours prior to performing the ONE-StepTM Luciferase assay. TL1A is the sole ligand for death receptor 3 (DR3 or TNFRSF25), a TNF receptor family member that induces apoptosis upon T cell activation. Dose-response to TL1A and TNFα in the TL1A Responsive Luciferase Reporter Jurkat cell line. Neutralization levels rose with concentration, with an off-target antibody serving as a 吉满生物H_TNFSF15 (TL1A) Reporter Cell Line报告基因细胞系，是一种Luciferase报告基因细胞系。 通过加入TL1A的拮抗型抗体，阻断由TL1A激活的DR3下游信号，从而可以通过测定荧光信号确定荧光素酶（Luciferase）的表达。 This cell line has been validated to be responsive to TL1A and TNF-α stimulation. HEK-Blue™ TL1A cells are designed to monitor human TL1A-induced NF-κB/AP1 stimulation or inhibition. By adding TL1A antagonistic antibodies to block the downstream signaling activated by TL1A, the expression of firefly luciferase can be determined via measuring the fluorescence signal. Expression of the firefly luciferase reporter is driven by NF-kB response . This cell line has been validated to be responsive to TL1A and TNF-α stimulation. This cell line can be used for screening antagonistic drugs targeting TL1A. Mycoplasma Testing The cell line has been screened to confirm the absence of Mycoplasma species. The cell line is a TL1A-responsive DR3/NF-κB luciferase reporter Jurkat cell line expressing firefly luciferase under the control of an NF-κB response element with stable expression of human DR3. 3). Description TNF-like ligand 1A (TL1A)-Responsive Luciferase Reporter Jurkat Cell Line is a TL1A-responsive DR3/NF-kB luciferase reporter Jurkat cell line expressing firefly luciferase under the control of an NF-kB response element, and with stable expression of human DR3 (death receptor 3; TNFRSF25; NM_003790. The results are shown as fold induction of luciferase reporter expression, determined by comparing luciferase activity of stimulated cells against the activity InvivoGen's cytokine reporter cells comprise an expanding family of engineered cell lines designed to provide a simple, rapid and reliable method to monitor the activation of signaling pathways induced by key cytokines. In T cells, it functions as a co-stimulator, boosting IL-2 reactivity and pro-inflammatory cytokine secretion. Expression of the firefly luciferase gene is driven by NF-kB response elements Human DR3 (TL1A receptor) (Luc) Reporter Cell Line ,we constructed a luciferase reporter cell line that co-expresses luciferase and target protein by stimulation. Figure 3. This colorimetric cytokine bioassay can be used to screen activatory or inhibitory molecules, such as engineered cytokines and neutralizing antibodies, respectively. Feb 19, 2024 · Activation of the NF-kB signaling pathway by the DR3 ligand TL1A (TNF-like Protein 1A; TNFSF15) can be monitored by measuring luciferase activity. zoqgqp re0 ht8e0k vanz2 yq0 dmehd ri ryk abg3l xno